Fig. 4: RBMS1 promotes the migration and invasion of GC cells in vitro.
From: RBMS1 promotes gastric cancer metastasis through autocrine IL-6/JAK2/STAT3 signaling
A SGC-7901 and HGC-27 cells transfected with RBMS1-knockdown and RBMS1-overexpression lentivirus were assessed under a bright-field microscope (left panels: magnification, ×10) and green fluorescence microscope (middle panels: magnification, ×10). Scale bars = 100 μm. B The protein expression of RBMS1 in SGC-7901 and HGC-27 cells was examined by western blot analysis after knocking down or overexpressing RBMS1. C MTT assay indicated the viability of RBMS1 knockdown or RBMS1-overexpressing SGC-7901 and HGC-27 cells. D A wound-healing assay was performed in SGC-7901 and HGC-27 cells with knockdown or overexpression of RBMS1. Representative images at the indicated time points are shown (magnification ×20). E Quantitative analysis of the migration area was performed for SGC-7901 and HGC-27 cells using ImageJ software. F Representative images of SGC-7901 and HGC-27 cells transfected with control or RBMS1 knockdown or overexpression vectors in migration and invasion assays. Scale bars = 100 μm. G Bar graphs show the statistics for cell counts. H Western blot assay was performed to detect EMT markers in SGC-7901 and HGC-27 cells with knockdown or overexpression of RBMS1. *P < 0.05, **P < 0.01, ***P < 0.001. NC negative control, KD knockdown, OE overexpression.
