Fig. 7: RBMS1 promotes the migration and invasion of GC cells through autocrine IL-6/JAK2/STAT3 signaling.
From: RBMS1 promotes gastric cancer metastasis through autocrine IL-6/JAK2/STAT3 signaling
A The correlation between RBMS1 and IL-6 in GC in the CCLE and TCGA databases. B ELISA showed the change in IL-6 expression after knocking down or overexpressing RBMS1 in SGC-7901 (B) and HGC-27 cells (C). D Western blot analysis indicated the differential levels of STAT3, p-STAT3, ERK, p-ERK, AKT, p-AKT, JAK2, and p-JAK2 after knocking down or overexpressing RBMS1 in SGC-7901 and HGC-27 cells. GAPDH was used as a loading control in the western blot analysis. E Transwell assays showed the change in migration and invasion after adding IL-6 to SGC-7901 and HGC-27 cells with RBMS1 knockdown. Scale bars = 100 μm. F Transwell assays showed the changes in migration and invasion after IL-6 neutralizing antibody treatment in SGC-7901 and HGC-27 cells overexpressing RBMS1. Scale bars = 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001. NC negative control, KD knockdown, OE overexpression; magnification, ×20.
