Fig. 8: RBMS1 interacts with MYC to regulate cytokine transactivation.
From: RBMS1 promotes gastric cancer metastasis through autocrine IL-6/JAK2/STAT3 signaling
A The three-line table shows the recruitment and binding coefficients of transcription factors interacting with RBMS1 in the two gene promoter regions. Enrichment scores were formed by the GeneHancer website, and interaction scores were obtained from the STRING website. B, C Co-IP assay showed the interaction between RBMS1 and MYC in SGC-7901 (B) and HGC-27 (C) cells. Input represents 5% of the total cell extract used for each immunoprecipitation; D Potential binding sites and sequences of IL-6 and TNF-α promoter MYC predicted from NCBI website; E–H ChIP assay was performed using the indicated antibodies in two kinds of cells with knocking down RBMS1 expression by siRBMS1 and analyzed by qPCR. The data are representative of more than two independent experiments. **P < 0.01, ***P < 0.001, ****P < 0.0001. NS not significant, NC negative control, KD knockdown, OE overexpression.
