Fig. 3: Influence of HDACi on TMZ-induced protein expression.

A Volcano Plot of data obtained by quantitative mass spectrometry comparing LN229 cells exposed to TMZ and TMZ/MS-275 for 72 h. Approximately 4000 proteins were detected in quadruple samples, of which 196 were significantly altered by MS-275. B Influence of MS-275 on the expression of RAD18 in glioma cells. The RAD18 protein expression of glioma cells (LN229, A172, U87MG, LN308) upon treatment was determined by Western blotting. Cells were exposed to MS-275 (1.5 µM), TMZ (50 µM) and TMZ/MS-275 and the assay was performed 72 h later. C Influence of MERCK60 on the expression of RAD18 in LN229 and A172 cells as determined by western blotting. Cells were exposed to MS-275 (1.5 µM) or MERCK60 (1.5 µM) and the assay was performed 72 h later. D Influence of HDAC1 or HDAC2 on the expression of RAD18 in LN229 cells as determined by western blotting. HDAC1 and HDAC2 were knocked down using RNAi and the assay was performed 144 h later. For B, C, and D ß-ACTIN served as loading control.