Fig. 5: Effect of RAD18 downregulation by MS-275 on DNA replication and cell cycle progression. | Cell Death & Disease

Fig. 5: Effect of RAD18 downregulation by MS-275 on DNA replication and cell cycle progression.

From: Class I HDAC overexpression promotes temozolomide resistance in glioma cells by regulating RAD18 expression

Fig. 5

A Quantification of EdU incorporation in LN229 and A172 cells exposed to indicated drugs. EdU incorporation and cell cycle distribution were determined by flow cytometric analysis. B Overexpressed RAD18 prevents TMZ-induced S-phase accumulation independent of MS-275. Cells were transiently transfected with RAD18-EGFP plasmid and cell cycle distribution was determined by Sub-G1 analysis 120 h after drug exposure. P ≤ 0.05; **P ≤ 0.01 and ***P ≤ 0.001. Indicated significances result from an unpaired t-test with Welch’s correction. For A and B cells were exposed to MS-275 (1.5 µM), TMZ (50 µM) and TMZ/MS-275 and assays were performed 72 h and 120 h later, respectively.

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