Fig. 5: Exhaustion of HSPC but not MSC in ALL PDX mice.

Mice were injected with 5 × 10⁵ cells (T-ALL) or 10⁵ cells (B-ALL) and murine Sca1⁺ cells were recovered when mice were developing ALL disease (day 35). A The number of Sca1⁺ cells recovered from the BM of control NSG mice and PDX mice developing T-ALL or B-ALL. Data are shown as means ± SD; n = 5 mice. B Analysis of Sca1⁺ cells by flow cytometry showing loss of hematopoietic progenitors (CD45⁺ c-Kit⁺) and HSC (SLAM; CD150⁺ CD48⁻) in T-ALL and B-ALL PDX mice. Data are shown as means ± SD; n = 5 mice. C 25% of the Sca1⁺ cells were cultured in methylcellulose media and hematopoietic CFU were observed at day 7; scale bar represents 5 mm. Data are shown as means ± SD; n = 3 mice. D 75% of the Sca1⁺ cells were cultured in media for growth of mesenchymal CFU for 10 days, scale bar represents 1 cm. Data are shown as means ± SD; n = 3 mice. On this figure, P value is measured by one-way Anova with Tukey’s multiple comparison test; ***P < 0.001; ****P < 0.0001; ns non-significant.