Fig. 7: T-ALL and B-ALL EVs are enriched with metabolites and cholesterol. | Cell Death & Disease

Fig. 7: T-ALL and B-ALL EVs are enriched with metabolites and cholesterol.

From: Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells

Fig. 7

A UPLC-LC-MS/MS data showing the quantity of all lipids (nmol/1010 particles) identified in T-ALL EVs and B-ALL EVs compared with NSG EVs. Triacylglycerides (TG), diacylglycerides (DG), ceramides (Cer), sphingomyelins (SM), phospho-cholines (PC), phospho-ethanolamines (PE), phospho-glycerols (PG), phospho-inositols (PI). B Distribution of each family of lipids in T-ALL EVs, B-ALL EVs and NSG EVs. C Data showing increased quantities of other amino acids measured by UPLC LC-MS/MS in T-ALL EVs and B-ALL EVs when compared with NSG EVs. D Data showing how cholesterol was highly present in T-ALL EVs and B-ALL EVs when compared with NSG EVs. ALL EVs also show elevated levels of ATP and glucose; n = 4 mice. P value measured by one-way Anova with Tukey’s multiple comparison test; **P < 0.01; ****P < 0.0001. E Flow cytometry data showing a relevant loss of quiescent cells (G0) with the lipid fractions isolated from T-ALL or B-ALL EVs when compared with lipids isolated from control NSG mice. Data are shown as means ± SD; n = 4 mice. P value measured by one-way Anova with Tukey’s multiple comparison test; ***P < 0.001; ns, non-significant.

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