Fig. 8: T-ALL and B-ALL EVs activate mitochondrial activity in HSPC.
A Extracellular acid production measured with the Seahorse on Lin− cells after 24 h of treatment with T-ALL EVs, B-ALL EVs, or NSG EVs (2 × 109 particles); n = 4 mice, in technical triplicates. B Oxygen consumption rate (OCR) measured with the Seahorse on Lin− cells after 24 h of treatment with T-ALL EVs and B-ALL EVs, compared with NSG EVs. Data showing that the basal and maximal respiration were increased following 24 h of Lin− cell exposure to ALL EVs (2 × 109 particles). Basal respiration corresponds to means of OCR measured for points 1–4 and maximal respiration for points 8–10. Oligomycin (1.5 μM), FCCP (1 μM), antimycin A, rotenone, (AA/Rot, 0.5 μM/0.5 μM); n = 4 mice, in technical triplicates. C Flow cytometry showing increased detection of the mitochondrial membrane potential states following the exposure of Lin− cells to ALL EVs (2 × 109 particles) for 24 h. Cells were treated with TMRM for 30 min before flow cytometry recording. Data performed on Lin− cells and gating on LK, LSK and LSK CD34− cells. Example of plot for TMRM recording with dashed line corresponding to mean fluorescence intensity (MFI) for Mock. On this figure, (A, B) data are shown as means ± SD; n = 4 mice in technical triplicates, (C) data are shown as means ± SD; n = 4 mice. P value measured by one-way Anova with Tukey’s multiple comparison test; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, non-significant.
