Fig. 2: The inflammation is increased within colon tumors of Tipe^−/− mice.

A Flow cytometic dot plot analysis of CD45⁺F4/80⁺CD11b⁺ cells and CD45⁺Ly-6G⁺CD11b⁺ cells in colon tumor tissues from WT (n = 5) and Tipe^−/− (n = 5) mice on day 90 of the CAC model. B Quantification of CD45⁺F4/80⁺CD11b⁺ cells and CD45⁺Ly-6G⁺CD11b⁺ cells in colon tumor tissues as described in (A). C Western blot analysis of p-P65 and p-STAT3 in the colon tumor tissues of WT (n = 4) and Tipe^−/− (n = 4) mice on day 90 of the CAC model. Blots represent data from the same biological samples run in parallel. D Quantification of inflammatory genes Il6, Il17a, Il22, Il23p19 and S100a8 mRNA expression by quantitative real-time PCR in the colon tumor tissues of WT (n = 5) and Tipe^−/− (n = 5) mice on day 90 of the CAC model. Data are presented as mean ± SEM and are representative of three independent experiments. Student’s t test (B, D), *p < 0.05, **p < 0.01.