Fig. 1: Generation of patient-derived organoids from colorectal cancer. | Cell Death & Disease

Fig. 1: Generation of patient-derived organoids from colorectal cancer.

From: KLF5 inhibition overcomes oxaliplatin resistance in patient-derived colorectal cancer organoids by restoring apoptotic response

Fig. 1

A Schematic diagram for the preparation of CRC PDOs. Only tumor samples can form PDOs, and normal cells died in a short time. Scale, 100 μm. B The appearance of CRC PDO in suspension culture or 3D culture. Scale, 100 μm. C, D Flow cytometry showed that the CRC PDOs were highly purified cancer cell clusters. E E-cadherin (green) and β-catenin (red) staining of PDOs. E-cadherin was located on the cell membrane and linked to β-catenin. Scale, 100 μm. F PDOs expressed EpCAM and Ki-67. Scale, 200 μm. Data represent the mean ± SD.

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