Fig. 2: Mammary gland regeneration in vivo after mammary stem cell transplantation.
From: Evolution of gene expression signature in mammary gland stem cells from neonatal to old mice
A, B Whole-mount staining of the mammary branching tree structures regenerated from the mammary stem cells isolated from mice at days 4, 20, 40, 60, 180, and 360 after birth, respectively. Same volume of PBS was used for injection as a negative control (A). Total amount of 500 MaSCs at each time point was transplanted into the epithelium-free mammary fat pad between the inguinal lymph node and the midline of 3-week-old female mice (B). C Quantitative analysis of the primary branches extending from the lymph node to end buds. D Quantitative analysis of the side-branches extending from the primary branches to end buds. Data are presented as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01.
