Fig. 2: Knockdown of KDM5B inhibits the function of Ewing sarcoma cells.
From: KDM5B promotes tumorigenesis of Ewing sarcoma via FBXW7/CCNE1 axis
A Western blot analysis of KDM5B expression in A673 and RDES cells after shKDM5B_NC, shKDM5B_1, shKDM5B_2, and shKDM5B_3 treatment. B Growth curves of A673 and RDES cells after shKDM5B treatment. C Left panel: Representative images of colony formation assays of A673 and RDES cells after shKDM5B treatment. Right panel: Histogram of three biological independent experiments of colony formation assays. D Left panel: Representative images of cell apoptosis analysis of A673 and RDES cells after shKDM5B treatment using flow cytometry. Right panel: Histogram of three biological independent experiments of cell apoptosis analysis. E Left panel: Representative images of cell cycle analysis of A673 and RDES cells after shKDM5B treatment using flow cytometry. Right panel: Histogram of three biological independent experiments of cell cycle analysis. Error bars in B, C, D, E indicate standard deviation of three biological independent experiments. P values in B are determined by two‐way ANOVA test. P‐values in C, D are determined by two‐tailed unpaired Student’s t test. *Above the bars indicates significant difference; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
