Fig. 3: KDM5B inhibitor AS-8351 suppresses the function of Ewing sarcoma cells.
From: KDM5B promotes tumorigenesis of Ewing sarcoma via FBXW7/CCNE1 axis
A Western blot analysis of KDM5B and H3K4me3 expression in A673 and RDES cells after AS-8351 treatment. B Cell viability assay of AS-8351 against A673 and RDES cell lines. Statistical chart showing the IC50 values of AS-8351 against A673 and RDES cell lines at different time points. C Growth curves of A673 and RDES cells after AS-8351 treatment. D Left panel: Representative images of colony formation assays of A673 and RDES cells after AS-8351 treatment. Right panel: Histogram of three biological independent experiments of colony formation assays. E Up panel: Representative images of cell apoptosis analysis of A673 and RDES cells after AS-8351 treatment using flow cytometry. Down panel: Histogram of three biological independent experiments of cell apoptosis analysis. F Up panel: Representative images of cell cycle analysis of A673 and RDES cells after AS-8351 treatment using flow cytometry. Down panel: Histogram of three biological independent experiments of cell cycle analysis. Error bars in B, C, D, E, F indicate standard deviation of three biological independent experiments. P values in C are determined by two‐way ANOVA test. P values in D, E are determined by two‐tailed unpaired Student’s t test. *Above the bars indicates significant difference; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
