Fig. 1: Mcl-1 levels influence cytarabine (CYT)-induced growth inhibition and DNA damage in AML cells. | Cell Death & Disease

Fig. 1: Mcl-1 levels influence cytarabine (CYT)-induced growth inhibition and DNA damage in AML cells.

From: Artesunate improves venetoclax plus cytarabine AML cell targeting by regulating the Noxa/Bim/Mcl-1/p-Chk1 axis

Fig. 1

A Five AML cell lines were treated with 0.05–0.8 μM cytarabine for 72 h. Cell growth inhibition was measured by counting cell number and compared to the untreated group. B THP-1 and MOLM-13 cells were treated with cytarabine at the indicated concentrations and times. Cell cycle distribution assessed by FACS after PI staining. C Apoptotic cells determined by FACS with staining of Annexin V/PI. D Basal levels of Mcl-1, Bcl-2, Bim, and Noxa in five AML cell lines. E Relative levels of the indicated proteins of THP-1 and MOLM-13 cells after treatment with cytarabine determined by Western blotting. F THP-1 cells were transfected with two pairs of MCL1 siRNAs for 24 h, then treated with 8 μM cytarabine for 24 h, and the levels of γ-H2A.X and p-Chk1 were determined by Western blotting. The column graphs are the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 comparing to the control group by t-test.

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