Fig. 2: H. pylori upregulated the expression of circMAN1A2 in gastric cancer cells.
From: CircMAN1A2 is upregulated by Helicobacter pylori and promotes development of gastric cancer
a After Hp26695-infected AGS cells were infected at an MOI = 100:1 for 12 h, the expression of circRNAs was detected by qRT–PCR. The control group used PBS for the same amount of time. b qRT–PCR analysis of circMAN1A2 expression in Hp+ gastritis tissues (n = 47) and Hp− gastritis tissues (n = 52). (Mann–Whitney U). c After Hp26695 infected AGS and BGC823 cells at a MOI = 100:1 for different times (0, 3, 6, 12 and 24 h), the relative expression of circMAN1A2 was assessed by qRT–PCR. d. After Hp26695 infected AGS and BGC823 cells with different MOIs (0, 50, 100, and 150) for 12 h, the expression of circMAN1A2 was detected by qRT–PCR. e Western blot was used to detect the expression of CagA protein in AGS and BGC823 cells after Hp26695-CagA+ and Hp26695-CagA- infection for 24 h. f After Hp26695-CagA- infected AGS and BGC823 cells at a MOI = 100:1 for different times (0, 3, 6, 12 and 24 h), the expression of circMAN1A2 was detected by qRT–PCR. g CagA protein in AGS and BGC823 cells transfected with the PcDNA3.1-CagA plasmid and PcDNA3.1 plasmid was analysed by Western blot. h qRT–PCR detected the expression of circMAN1A2 in AGS and BGC823 cells transfected with the PcDNA3.1-CagA plasmid and PcDNA3.1 plasmid. i After Hp26695 and heat-killed Hp26695 infected AGS and BGC823 cells at a MOI = 100:1 for 12 h, the expression of circMAN1A2 was assessed by qRT–PCR. The control group used PBS. *P < 0.05, **P < 0.01, ***P < 0.001.
