Fig. 3: CircMAN1A2 could promote the proliferation, migration and invasion of gastric cancer cells.
From: CircMAN1A2 is upregulated by Helicobacter pylori and promotes development of gastric cancer
a qRT–PCR analysis of circMAN1A2 in the human immortalized gastric epithelial cell line GES-1 and gastric cancer cell lines MGC803, AGS, BGC823, SGC7901 and HGC27. b The interference efficiency of circMAN1A2 siRNAs was detected by qRT–PCR in AGS cells and BGC823 cells. c The proliferation ability of AGS and BGC823 cells transfected with circMAN1A2 siRNAs was detected by CCK-8 assay at 5 time points (24, 48, 72, 96 and 120 h). d The colony formation test was used to detect the clonogenic ability of AGS and BGC823 cells transfected with circMAN1A2 siRNAs. e The migration ability of AGS and BGC823 cells transfected with circMAN1A2 siRNAs was detected by Transwell assays without Matrigel. f The invasion ability of AGS and BGC823 cells transfected with circMAN1A2 siRNAs was measured by Transwell assays with Matrigel. *P < 0.05, **P < 0.01, ***P < 0.001.
