Fig. 1: Short-term and long-term proliferation inhibitory effects of penfluridol on renal cell carcinoma (RCC). | Cell Death & Disease

Fig. 1: Short-term and long-term proliferation inhibitory effects of penfluridol on renal cell carcinoma (RCC).

From: Targeting DRD2 by the antipsychotic drug, penfluridol, retards growth of renal cell carcinoma via inducing stemness inhibition and autophagy-mediated apoptosis

Fig. 1

A Four RCC cell lines including papillary type (ACHN) and clear cell type (Caki-1, A498, and 786-O), were treated with the indicated concentrations of penfluridol (1.25, 2.5, 5, 10 and 20 μM) or dimethyl sulfoxide (vehicle control) for 24 h, and an MTT assay was performed to determine cell viability. Data are presented as the mean ± standard deviation (SD) from three independent experiments. Values of the half maximal inhibitory concentration (IC50) of these cells are shown in the lower panel. B and C Colony-forming abilities of 786-O and A498 cells after treating cells with the indicated concentrations of penfluridol for 7 days (B) or 24 h (C). Upper panels of B, C: representative photomicrographs. Lower panels of B, C: values are presented as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 compared to those of the vehicle group. ns: not significant. D After treatment of A498, 786-O, or Caki-1 cells with 5 μM penfluridol and/or 20 μM sunitinib/sutent for 24 h, the proliferative abilities of these ccRCC cells were determined by a CCK-8 assay. **p < 0.01 and ***p < 0.001 compared to those of the vehicle group. ##p < 0.01 and ###p < 0.001 compared to those of the sunitinib/sutent only group.

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