Fig. 1: Depletion of p54nrb lowers the viability of tumor cells. | Cell Death & Disease

Fig. 1: Depletion of p54nrb lowers the viability of tumor cells.

From: The caspase-2 substrate p54nrb exhibits a multifaceted role in tumor cell death susceptibility via gene regulatory functions

Fig. 1

P54nrb expression level in healthy tissue versus tumor tissue A from cervix carcinoma, B from colon carcinoma, and C from melanoma patients. Values were obtained from Oncomine database. To test significance Student´s t test was performed. ****p < 0.0001. Immunoblot of p54nrb-level in shRNA-control and shRNA-p54nrb#1 knockdown cells D from DLD-1 and E from SK-MEL. Counts of colonies of 3D soft agar tumor growth assay of shRNA-control and shRNA-p54nrb#1 cells F from DLD-1 and G from SK-MEL. 1000 cells/well were seeded and grown for 3 weeks and stained with 0.1% crystal-violet. Student´s t test was performed. *p < 0.05, n = 3 (G). Representative wells of 3D soft agar tumor growth assay of shRNA-control and shRNA-p54nrb#1 cells H from DLD-1 and I from SK-MEL cells. J Schematic representation of protein domains of p54nrb (also: NoNO): H and Q rich domain (HQ), RNA recognition motif (RRM), NonA/paraspeckle domain (NOPS), coiled-coil domain (COIL), and proline-rich region (P). Brackets indicate the region for RNA, DNA, and protein binding.

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