Fig. 2: Depletion of p54nrb leads to increased cell death susceptibility. | Cell Death & Disease

Fig. 2: Depletion of p54nrb leads to increased cell death susceptibility.

From: The caspase-2 substrate p54nrb exhibits a multifaceted role in tumor cell death susceptibility via gene regulatory functions

Fig. 2

A Immunoblot of p54nrb-level in shRNA-control and shRNA-p54nrb#3 knock down HeLa cells. B Flow cytometry of HeLa shRNA-control and shRNA-p54nrb#3 cells at 24 h after treatment with 10 µM etoposide (Eto) and 240 ng/ml human recombinant TRAIL (TRAIL). Cell viability was measured by detection and totaling of Annexin-V single positive and Annexin-V and propidium-iodide- double positive cells. The cells not falling to either of these categories were considered as viable cells. Significance was calculated with Student´s t test, *p < 0.05, n = 3. C Flow cytometry of HeLa shRNA-control and shRNA-p54nrb#3 cells at 24 h after treatment with 10 µg/ml Doxorubicin (DOX). Cell viability was measured by detection and totaling of Annexin-V single positive and Annexin-V and Sytox Blue- double-positive cells. The cells not falling to either of these categories were considered as viable cells. Significance was calculated with Student´s t test, **p < 0.01, n = 3.

Back to article page