Fig. 4: P54nrb is cleaved upon apoptosis stimulation depending on the presence of caspase-2.

A Immunoblot of p54nrb cleavage in HeLa cells at 24 h after treatment with DMSO or 20 µM Z-VDVAD-fmk (-1 h) and 250 nM staurosporine (STS). B Immunoblot of p54nrb cleavage and caspase expression in HeLa cells. HeLa cells were transfected either with 300 or 1000 ng/ml pcDNA3-caspase-2-Flag or pcDNA3-caspase-3-myc, and 24 h later were treated either with DMSO as vehicle control or 250 nM STS. The samples were harvested for Western blot at 24 h. C Immunoblot of p54nrb cleavage and caspase-2 in DLD-1 CRISPR-control or DLD-1 CRISPR–caspase-2 cells at 24 h after treatment with DMSO, 10 µM or 50 µM Eto. D Immunoblot of p54nrb cleavage and caspase-2 in HeLa shRNA-control or HeLa shRNA-caspase-2 cells at 24 h after treatment with DMSO, 250 nM STS, 1 µM STS or 10 µM Eto. E Immunoblot of p54nrb cleavage and caspase-2 in HeLa shRNA-control or HeLa shRNA-caspase-2 cells at 1, 3, 6, and 10 h after treatment with 1 µM STS. DMSO was used as vehicle control. F Immunoblot of p54nrb and caspase-2 of nuclear and cytoplasmic fraction of HeLa cells. Lamin-A was detected as nuclear marker.