Fig. 7: The effect of E2F1-miR-532 loop on GC tumor growth in vivo.
From: The miR-532-E2F1 feedback loop contributes to gastric cancer progression
A A schematic diagram illustrating the experimental design. MKN-45 cells overexpressing miR-532, E2F1 or miR-532 plus E2F1 were implanted subcutaneously into nude mice (3 mice per group) and tumor growth was evaluated on day 25 after implantation. B Images of tumors from GC mice. C Tumor volume curves. D Tumour weights. E Western blot analysis of E2F1, ASK1 and TXNIP levels in GC xenografted tumors. F IHC staining for Ki-67, E2F1 and Cleaved caspase-3 and IF staining for 53BP1 and γ-H2AX in tumors from four groups. *P < 0.05; **P < 0.01; ***P < 0.001.
