Fig. 1: Insulin receptor expression in the mouse retina.
From: Insulin receptor activation by proinsulin preserves synapses and vision in retinitis pigmentosa
A RT-PCR of WT and rd10 mouse retinas at the indicated ages, and WT adult brain regions and peripheral tissues. Forward and reverse PCR primers corresponded to exons 10 and 12, respectively, of Insr. CB, cerebellum; BS, brain stem; TE, remainder of telencephalon and diencephalon; OB, olfactory bulb; M, muscle; L, liver; A, adipose tissue. ß-actin was used as a loading control. B–F Representative images of P21 retinal sections from WT mice. B Image of a retinal section immunostained for INSR (green). Nuclei are stained with DAPI (blue). C–F Magnified image of the OPL showing double (C–E) or triple (F) immunostaining for the indicated markers. In F, asterisks indicate cell bodies, arrows axons and arrow heads dendrites of horizontal cells. ONL Outer nuclear layer, OPL Outer plexiform layer, INL Inner nuclear layer, IPL Inner plexiform layer; NFL Nerve fiber layer. Scale bars: 90 μm (B), 45 μm (C–E) and 13 μm (F).
