Fig. 2: Downregulation of insulin receptor expression in the rd10 mouse retina.
From: Insulin receptor activation by proinsulin preserves synapses and vision in retinitis pigmentosa
A, D Representative images of P21 (A) and P23 (D) retinal sections from WT and rd10 mice co-immunostained for INSR (green) and neurofilament-M (NF-M, red). Nuclei are stained with DAPI (blue). ONL Outer nuclear layer, OPL Outer plexiform layer, INL Inner nuclear layer, IPL Inner plexiform layer, NFL Nerve fiber layer. Scale bar: 66 μm. B, E Quantification of the area of INSR-positive staining in P21 (B) and P23 (E) WT and rd10 retinal sections. The area corresponding to INSR immunostaining was normalized to that of neurofilament-M within the same region to correct for potential variations among retinal sections, and to the INSR/NF-M ratio in corresponding WT sections ( =1.0). C, F Quantification of the area of NF-M-positive staining in P21 (C) and P23 (F) WT and rd10 retinal sections expressed relative to WT levels ( =1.0). Data are presented as the mean + SEM. n = 4–5 mice, 4 images per retina. *p ≤ 0.05 (unpaired-T test in B and C and unpaired-T test with Welch’s correction in E and F).
