Fig. 2: Stathmin regulates cell migration through microtubule modulation. | Cell Death & Disease

Fig. 2: Stathmin regulates cell migration through microtubule modulation.

From: HMGA1 positively regulates the microtubule-destabilizing protein stathmin promoting motility in TNBC cells and decreasing tumour sensitivity to paclitaxel

Fig. 2

A Western blot analysis of tubulin protein levels in soluble-tubulin (S) and polymerized-tubulin (P) fractions after microtubule separation assay performed in MDA-MB-231 cells silenced for stathmin expression (siSTMN), control (siCTRL), or taxol (a drug that stabilizes microtubules counteracting their disassembling) as positive control of tubulin polymerization. Densitometry analysis of tubulin expression (normalized on total tubulin amount of each sample) is reported in the bottom graphs and presented as the mean (±SD) of n = 3 replicates. B Quantification of the trans-well migration assay performed in MDA-MB-231 cells upon silencing of stathmin (siSTMN) and 1 h of nocodazole treatment. The results are presented as the mean of the percentage of migrated cells with respect to control (n = 3). Representative pictures are shown on the right of the graph. Stathmin western blot analyses are reported at the bottom.

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