Fig. 5: CTSS is a direct transcriptional target of MEOX2.
From: MEOX2-mediated regulation of Cathepsin S promotes cell proliferation and motility in glioma
A Volcano diagrams display differential genes expression in SNB19 cells with MEOX2 silencing (left), and U87 cells with MEOX2 overexpression (right). B Venn diagram depicts the overlap genes between upregulation in U87 cells and downregulation in SNB19 cells, or between upregulation in SNB19 cells and downregulation in U87 cells. C RT-PCR analysis was performed to determine the relative mRNA levels of CTSS, ANGPTL4, IL11RA in SNB19 cells with MEOX2 inhibition and U87 cells with MEOX2 overexpression or control cells. D Immunoblot of CTSS protein levels in SNB19, U118, GSC23 cells transfected with MEOX2 siRNAs or Scramble siRNA, U87, GSC91 cells which were overexpressed with MEOX2 or EV. E The correlation between MEOX2 and CTSS expression in gliomas from TCGA database. F The mRNA expression of CTSS in gliomas with different WHO grades from TCGA and CGGA databases. G Overall survival (OS) curves of patients with glioma from TCGA and CGGA databases stratified by CTSS expression. H The DNA-binding motif of MEOX2. I Schematic depicting the detailed information of the potential MEOX2-binding sites on CTSS promoter are shown. J ChIP-qPCR analysis of MEOX2 binding on CTSS promoter in SNB19 and U118 cells. K Luciferase reporter assays were utilized to detect the transcriptional activity of full length or different clipped forms of CTSS promoter in SNB19 and U118 cells which were transfected with MEOX2 siRNA or Scramble siRNA. ns: no significant, # p < 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
