Fig. 6: CTSS depletion retards glioma cell proliferation and motility.
From: MEOX2-mediated regulation of Cathepsin S promotes cell proliferation and motility in glioma
A Western blotting analysis of CTSS expression in CTSS-silenced SNB19, U118, GSC23 cells or control cells. B Cell viability was measured in SNB19 and U118 cells with CTSS siRNA or Scramble siRNA transfection. C The self-renewal capability of CTSS knockdown GSC23 cells or control cells was determined by limiting dilution assay. D Representative images of sphere formation capability of GSC23 cells with or without CTSS inhibition. Bar = 100 µm. E Representative EdU fluorescence images of control, siCTSS-treated SNB19, U118, GSC23 cells. Bar = 50 µm. F Quantification of the EdU positive cells rate. G Wound-healing assay was performed to measure the migration ability of CTSS-knockdown SNB19 and U118 cells or control cells. Bar = 400 µm. H Quantification of the wound healing rate. I Transwell assay was utilized to assess the invasion and migration capacities in SNB19, U118 cells with CTSS inhibition or control cells. Bar = 100 µm. J Quantification of the invasive and migrated cells number. *p < 0.05, ***p < 0.001, ****p < 0.0001.
