Fig. 8: CTSS re-expression partially abrogates cell proliferation and motility inhibition caused by MEOX2 silencing in glioma.
From: MEOX2-mediated regulation of Cathepsin S promotes cell proliferation and motility in glioma
A Immunoblot analysis of CTSS expression in SNB19, U118, GSC23 cells treated with Scramble shRNA, shMEOX2, shMEOX2 + CTSS EV and shMEOX2 + CTSS OE. B Cell viability of SNB19 and U118 cells infected with Scramble shRNA, shMEOX2, shMEOX2 + CTSS EV and shMEOX2 + CTSS OE was evaluated by CCK-8 assay. C The self-renewal capability of GSC23 cells treated with Scramble shRNA, shMEOX2, shMEOX2 + CTSS EV and shMEOX2 + CTSS OE was assessed by limiting dilution assay. D Cell proliferation of SNB19, U118, GSC23 cells infected with Scramble shRNA, shMEOX2, shMEOX2 + CTSS EV and shMEOX2 + CTSS OE was determined by EdU assay. E Cell migration ability of SNB19 and U118 cells infected with Scramble shRNA, shMEOX2, shMEOX2 + CTSS EV and shMEOX2 + CTSS OE was evaluated by wound healing assay. F Cell invasion and migration capacities of SNB19, U118 and GSC23 cells treated with Scramble shRNA, shMEOX2, shMEOX2 + CTSS EV and shMEOX2 + CTSS OE was detected by transwell assays. G Control, shMEOX2, shMEOX2 + CTSS EV and shMEOX2 + CTSS OE-infected GSC23 cells were intracranially injected into nude mice, and representative HE images of xenograft tumors are shown. H Quantification of the tumor volumes. I Kaplan-Meier survival curve shows the survival times of GSC23 scramble, GSC23 shMEOX2, GSC23 shMEOX2 + CTSS EV and GSC23 shMEOX2 + CTSS OE xenograft mice. J IHC staining representative images show Ki67, MEOX2, CTSS, Vimentin and p-FAK expression in GSC23 scramble, GSC23 shMEOX2, GSC23 shMEOX2 + CTSS EV and GSC23 shMEOX2 + CTSS OE xenograft mice. Bar = 20 µm. * shMEOX2 treatment group vs control group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. # shMEOX2 + CTSS OE treatment group vs shMEOX2 + CTSS EV group, #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001.
