Fig. 3: POU6F1 represses the growth and aggressiveness of LUAD cells in vitro. | Cell Death & Disease

Fig. 3: POU6F1 represses the growth and aggressiveness of LUAD cells in vitro.

From: POU6F1 cooperates with RORA to suppress the proliferation of lung adenocarcinoma by downregulating HIF1A signaling pathway

Fig. 3

A, B Western blotting and real-time qRT-PCR assays revealing the POU6F1 expression in A549 and NCI-H1299 cells stably transfected with empty vector (mock) or POU6F1. C, D CCK8 and colony formation assays indicating cell viability of A549 and NCI-H1299 cells stably transfected with mock or POU6F1. E, F Representative images (left panel) and quantification (right panel) of soft agar and transwell assays revealing the migration ability of A549 and NCI-H1299 cells stably transfected with mock or POU6F1. G Cell cycle detection in A549 and NCI-H1299 cells transfected with empty vector (pCMV-HA) or pCMV-HA-POU6F1. H Immunofluorescence revealing cycle change of A549 and NCI-H1299 cells transfected with pCMV-HA or pCMV-HA-POU6F1, and those co-transfected with pRetroX-G1-Red and pRetroX-SG2M-Cyan vector. Scale bar: 100 μm. The student’s t-test compared the difference in B–G. *P < 0.05, **P < 0.01.

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