Fig. 5: POU6F1 inactivates HIF1A signaling pathway and transcriptionally regulates ENO1, PDK1, and PRKCB expression. | Cell Death & Disease

Fig. 5: POU6F1 inactivates HIF1A signaling pathway and transcriptionally regulates ENO1, PDK1, and PRKCB expression.

From: POU6F1 cooperates with RORA to suppress the proliferation of lung adenocarcinoma by downregulating HIF1A signaling pathway

Fig. 5

A Volcano plot showing differential expression genes (DEGs) in A549 cells stably transfected with POU6F1 compared with empty vector (mock). B Human KEGG pathway analysis of DEGs indicating participated pathways in A549 cells stably transfected with POU6F1 relative to mock. C Venn diagram revealing comprehensive analysis of genes, that were involved with the HIF1A signaling pathway and associated with LUAD progression in various status of death, tumor stage, and metastasis. D Western blotting assay showing the expression of HIF1A, ENO1, ENO2, GAPDH, PDK1, and PRKCB in A549 and NCI-H1299 cells stably transfected with mock or POU6F1. E Real-time qRT-PCR assay indicating the expression of HIF1A, ENO1, PDK1, and PRKCB in A549 cells stably transfected with mock or POU6F1. F Dual-luciferase assay indicating relative activity of HIF1A promoter in A549 and NCI-H1299 cells stably transfected with mock or POU6F1, and those treated with DMSO or ML228 (1.0 μmol/l). G Western blotting assay showing the expression of HIF1A in A549 and NCI-H1299 cells stably transfected with mock or POU6F1, and those treated with DMSO or MG132 (5 μmol/l) for 6 h. H Dual-luciferase assay showing relative activity of ENO1, PDK1, and PRKCB promoter in A549 and NCI-H1299 cells transfected with mock or POU6F1. I ChIP and qPCR assays indicating relative POU6F1 enrichment on ENO1, PDK1, and PRKCB promoter in A549 cells stably transfected with mock or POU6F1. Student t-test and ANOVA compared the difference in E, F and H, I. *P < 0.05, **P < 0.01.

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