Fig. 7: POU6F1 coordinates with RORA to inhibit LUAD progression. | Cell Death & Disease

Fig. 7: POU6F1 coordinates with RORA to inhibit LUAD progression.

From: POU6F1 cooperates with RORA to suppress the proliferation of lung adenocarcinoma by downregulating HIF1A signaling pathway

Fig. 7

A–D Dual-luciferase assay indicating the relative promoter activity of HIF1A, ENO1, PDK1, and PRKCB in A549 and NCI-H1299 cells transfected with mock, RORA, CRISPRi-Scb, or CRISPRi-POU6F1 #1. E, F Real-time qRT-PCR and western blotting assays showing the expression of POU6F1, RORA, HIF1A, ENO1, PDK1, and PRKCB in A549 cells transfected with mock, RORA, CRISPRi-Scb, or CRISPRi-POU6F1 #1. G, H Representative images (left panel) and quantification (right panel) of soft-agar (G) and transwell (H) assays indicating the growth and invasion of A549 cells transfected with mock, RORA, CRISPRi-Scb, or CRISPRi-POU6F1 #1. Student’s t-test and ANOVA compared the difference in A–E and G–H. *P < 0.05, **P < 0.01.

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