Fig. 1: Correlation of ApoE4 level in serum exosomes with thyroid hormones levels and cognitive performance in human subjects. | Cell Death & Disease

Fig. 1: Correlation of ApoE4 level in serum exosomes with thyroid hormones levels and cognitive performance in human subjects.

From: Ageing related thyroid deficiency increases brain-targeted transport of liver-derived ApoE4-laden exosomes leading to cognitive impairment

Fig. 1

A Enroled 60 carriers of ApoE4 allele included 15 adult healthy subjects, 15 old healthy subjects and 30 old AD patients. Collected serum was used to extract exosomes and measure TT3, FT3, TT4 and FT4. Participants cognitive abilities were assessed by MMSE and MoCA scoring. B Representative western blots of ApoE4 and exosomal specific marker CD81 and CD9 in the serum extracted exosomes and the supernatant from which exosomes precipitate was removed after ultracentrifuge. C Normalised intensities of ApoE4 by β-actin. D TT3, FT3, TT4 and FT4 in adult, old and AD groups. Correlation analyses between the ApoE4 level in serum exosomes and TH levels are shown in inserts at the right. E MMSE and MoCA scores; correlation analyses between the ApoE4 level in serum exosomes and these scores are shown in inserts at the right. F Multiple correlation analysis of the normalised ApoE4 in serum exosomes with the normalised MoCA scores and serum FT3 in adult group. The individual measured value was plotted and shown as mean ± SD. One-way ANOVA for comparisons including more than two groups; unpaired two-tailed t-test for two-group comparisons. *p < 0.05, **p < 0.01, ***p < 0.001.

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