Fig. 3: The effects of L-thy and ApoE4 inhibitor on the exosome transport of h-ApoE4 and cerebral oxidative stress in ApoE4-KI mice.

A Old mice were randomly intraperitoneally injected with normal saline (NS), 20 μg/kg/day L-thy or 200 mg/kg/day ApoE4 inhibitor PH-002 for 14 days; 2 months old mice randomly underwent thyroidectomy or sham surgery. Four weeks after surgery measurements were made. B TT3, FT3, TT4 and FT4 values are plotted as mean ± SD, n = 6. One-way ANOVA for comparisons including more than two groups; unpaired two-tailed t-test for two-group comparisons. As comparison with adult group, *p < 0.05, **p < 0.01, ***p < 0.001. C Representative Western blots of h-ApoE4 in the exosomes extracted from liver and serum, CD9 and CD81 were used as the specific markers of exosomes. D Expression ratio of h-ApoE4 and GAPDH are plotted as mean ± SD, n = 6. E Representative Western blots of ATP6AP1 in the exosomes extracted from liver and serum. F Ratio of ATP6AP1 normalised to GAPDH is plotted as mean ± SD, n = 6. One-way ANOVA for comparisons including more than two groups; unpaired two-tailed t-test for two-group comparisons. As comparison with adult group, *p < 0.05, **p < 0.01, ***p < 0.001. G, H Cholesterol and the ratio of GSH/GSSG in cortex and hippocampus measured by ELISA are plotted as mean ± SD, n = 6. I Representative Western blots of UCP4 in cortex and in hippocampus. J Ratio of UCP4 to β-actin is plotted as mean ± SD, n = 6. K mRNA expression of UCP4 measured by RT-PCR in cortex and in hippocampus, the relative ratio of UCP4 and GAPDH was normalised to adult group and plotted as mean ± SD, n = 6. L Mitochondrial ROS level in cortex and hippocampus are plotted as mean ± SD, n = 6. One-way ANOVA for comparisons including more than two groups; unpaired two-tailed t-test for two-group comparisons. As comparison with adult group, *p < 0.05, **p < 0.01, ***p < 0.001.