Fig. 2: Knockdown of SHANK1 inhibits the migration and invasion, and it increases the apoptosis of human lung cancer cell lines. | Cell Death & Disease

Fig. 2: Knockdown of SHANK1 inhibits the migration and invasion, and it increases the apoptosis of human lung cancer cell lines.

From: SHANK1 facilitates non-small cell lung cancer processes through modulating the ubiquitination of Klotho by interacting with MDM2

Fig. 2

A A549 and H1299 cells were transfected with SHANK1 shRNAs, followed by qRT-PCR assays to detect knockdown efficiency of siRNAs. B A549 cells were transfected with SHANK1 shRNAs, followed by WB assays to detect knockdown efficiency of siRNAs. C, D CCK8 assay showed that SHANK1 knockdown inhibited cell proliferation in A549 and H1299. E, F Clone formation assay showed that SHANK1 knockdown inhibited cell growth in A549, H1299, and SK-MES-1. G Transwell was performed to detect the migration and invasion of A549 cells. The number of migratory and invasive A549 cells was counted. H Transwell was performed to detect the migration and invasion of H1299 cells. The number of migratory and invasive H1299 cells was counted. *p < 0.05. I The apoptosis of A549 and H1299 cells was detected by flow cytometry. The column diagram shows the apoptosis cell number. J The apoptosis-related protein levels were detected by western blot. The column diagram shows the related expression levels of target genes. K The PI3K-AKT-related protein levels were detected by western blot. The column diagram shows the related expression levels of target genes. *p < 0.05.

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