Fig. 3: SHANK1 overexpression reduces the protein expression of KL in a dose-dependent manner.

A A549 cells were transfected with SHANK1, and relative protein levels of KL were detected by western blotting assay. Data shown are the mean ± SEM of three separate experiments (*p < 0.05, compared with the control group (0 ng), one-way ANOVA). B A549 cells were transfected with SHANK1, and relative mRNA levels of KL were detected by qRT-PCR. C, D Protein expression of SHANK1 and KL in NSCLC samples, as measured by WB, relative to that of Tubulin. T tumor. N normal. E, F A549 cells were transfected with empty vector or SHANK1 for 48 h and then serum-starved for the indicated times at 37 °C in the presence of cycloheximide (CHX, 20 μg/ml). KL was detected by anti-KL immunoblotting. Data shown are the mean ± SEM of three separate experiments (*p < 0.05, analysis of variance followed by post hoc tests). G, H A549 cells were transfected with SHANK1 vector for 48 h and then serum-starved for the indicated times at 37 °C in the presence of DMSO(1/1000), cycloheximide (CHX, 20 μg/ml) and MG132 (10 μM). KL was detected by anti-KL immunoblotting. Data shown are the mean ± SEM of three separate experiments (*p < 0.05, analysis of variance followed by post hoc tests).