Fig. 4: SHANK1 regulates ubiquitination of KL by interacting with KL. | Cell Death & Disease

Fig. 4: SHANK1 regulates ubiquitination of KL by interacting with KL.

From: SHANK1 facilitates non-small cell lung cancer processes through modulating the ubiquitination of Klotho by interacting with MDM2

Fig. 4

A A549 cells were transfected with SHANK1-myc and Flag-KL constructs and lysed by TNE buffer, followed by immunoprecipitation with anti-FLAG antibody. Western blotting analysis was performed. B Endogenous association of KL and SHANK1 in adult rat lung tissue was detected by co-immunoprecipitation. C Subcellular co-localization of endogenous KL and SHANK1 in A549 cells visualized by confocal microscopy. Staining was carried out with rabbit anti-SHANK1 (green) and mouse anti-KL (red) antibodies. D The ubiquitination of KL was detected by IP and western blotting with Ub antibody. E The relative Ub levels in (D) are shown (relative to vector expression level, the error bars indicate SEM for three experiments, **p < 0.01, determined by student’s t test).

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