Fig. 2: Lymphocyte heterogeneity after SCI. | Cell Death & Disease

Fig. 2: Lymphocyte heterogeneity after SCI.

From: Single-cell transcriptome analysis reveals the immune heterogeneity and the repopulation of microglia by Hif1α in mice after spinal cord injury

Fig. 2

A Flow cytometric analyses show the absolute number of lymphocytes per mouse after SCI. One-way ANOVA with Tukey’s multiple comparisons posttest, n = 11 mice in the 3 dpi group, n = 12 mice in the sham and 14 dpi groups, ****P < 0.0001. B Flow cytometric analyses show the proportion of αβ T cells, γδ T cells, B cells, NK cells, and NK T cells in lymphocytes per mouse after SCI, respectively. C Flow cytometric analyses show the absolute number of B cells per mouse after SCI. One-way ANOVA with Tukey’s multiple comparisons posttest, ****P < 0.0001. D An IHC image of a transverse section in the sham group shows the location of B cells (white arrows). Images indicate similar results from three independent mice. Scale bar: 50 μM. E, F Flow cytometric analyses show the absolute number of NK cells E and NK T cells F per mouse after SCI, respectively. One-way ANOVA with Tukey’s multiple comparisons posttest, ****P < 0.0001. G IHC images of sagittal sections show the location of NK cells at 14 dpi. A dashed white line outlines the lesion border. An enlarged image of a dashed white boxed area is displayed on the right. Images indicate similar results from three independent mice. Scale bar: 500 μM or 50 μM. V, veutro; D, dorsal; R, rostral; C, caudal. H, I Flow cytometric analyses show the absolute number of αβ T cells H and γδ T cells I per mouse after SCI, respectively. One-way ANOVA with Tukey’s multiple comparisons posttest, ****p < 0.0001; **P = 0.0025 (sham vs. 14 dpi); **P = 0.0031 (3 dpi vs. 14 dpi). J Flow cytometric analyses show the percentage of activated T cells at 3 and 14 dpi per mouse. Multiple unpaired two-tailed Student’s t-test, ****P < 0.0001. K IHC images of sagittal sections showing the location of αβ cells at 14 dpi. A dashed white line outlines the lesion border. Magnified images of the boxed area are displayed in the middle (asterisk) and right (triangle). Images indicate similar results from three independent mice. L IHC images of sagittal sections show the location of Th17 at 14 dpi. A dashed white line outlines the lesion border. Enlarged images of the boxed area are displayed in the middle (asterisk) and right (triangle). Images indicate similar results from three independent mice.

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