Fig. 2: TDP43 regulates AS of CD44.
From: TDP43 promotes stemness of breast cancer stem cells through CD44 variant splicing isoforms
A Schematic of CD44 pre-mRNA structure and TDP43-binding motif. Arrows indicate primers for qPCR (q) and RT-PCR (vx-F and v-R). B Binding of CD44 mRNA to TDP43 proteins detected by RIPA in HCC1806 cells. C, D qPCR analysis of changes in CD44v/s following TDP43 KD. E Semi-quantitative RT-PCR of CD44 isoforms upon TDP43 KD. The primer pairs (vx-F and v-R) are applied for CD44v amplification. e.g., v2 band is amplified using v2-F and v-R primers. F, G qPCR analysis of CD44v/s ratio following TDP43 OE. H qPCR analysis of CD44v/s changes at various TDP43 concentrations by doxycycline induction (μg/ml). Data are means ± SD.
