Fig. 3: SRSF3 maintains stemness of BCSCs.
From: TDP43 promotes stemness of breast cancer stem cells through CD44 variant splicing isoforms
A qPCR analysis of SRSF3 KD efficiency in HCC1806 and MDA-MB-231 cells. B, C Comparison of number of tumorspheres in MDA-MB-231 and HCC1806 cells upon SRSF3 KD. D, E Examination of stemness marker (SOX9, c-Myc, and OCT4) at RNA and protein levels upon SRSF3 KD in MDA-MB-231 and HCC1806 cells. F ALDH analysis upon SRSF3 KD in HCC1806 and MDA-MB-231 cells. G Western blotting analysis of SRSF3 protein levels in HCC1806 cells. H Tumorsphere assay upon SRSF3 OE. The unpaired t-test was used to compare the number of sphere among groups. I Flow cytometry analysis of ratios of CD44+/CD24−/low population after SRSF3 OE. J ALDH analysis following SRSF3 OE in HCC1806 and MDA-MB-231 cells. Data are means ± SD.
