Fig. 4: miR-1298-5p was sorted into exosomes via hnRNPA2B1. | Cell Death & Disease

Fig. 4: miR-1298-5p was sorted into exosomes via hnRNPA2B1.

From: The dual role of glioma exosomal microRNAs: glioma eliminates tumor suppressor miR-1298-5p via exosomes to promote immunosuppressive effects of MDSCs

Fig. 4

a Sequence motifs of hnRNPA2B1 binding site predicted by POSTAR2. b, c qRT-PCR assay analyzed the exo/cell ratio of miR-1298-5p in U87MG and P3 after knocking down hnRNPA2B1. d RNA pull-down and western blot with U87MG lysate confirmed that miR-1298-5p was associated with hnRNPA2B1. e RIP analysis using the anti-hnRNPA2B1 antibody revealed that miR-1298-5p interacted with hnRNPA2B1 in U87MG cells. The negative control, IgG. f Schematic graph of in vitro coculture system. g Internalization of Cy3-labeled miR-1298-5p by MDSCs. h, i hnRNPA2B1 was knocked down in U87MG. Internalization of Cy3-labeled miR-1298-5p by MDSCs was assessed by flow cytometry after coculture for 24 h. j, k Cultured MDSCs with U87MG and P3 knocking down hnRNPA2B1 and analyzed the ratio of CD14 + HLA-DR low/− MDSCs population using Flow cytometry assay. Statistical significance was determined using one-way ANOVA test (*P < 0.05; **P < 0.01; ***P < 0.001).

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