Fig. 6: The effects of miR-1298-5p overexpression in glioma could be partially attenuated by SETD7 overexpression. | Cell Death & Disease

Fig. 6: The effects of miR-1298-5p overexpression in glioma could be partially attenuated by SETD7 overexpression.

From: The dual role of glioma exosomal microRNAs: glioma eliminates tumor suppressor miR-1298-5p via exosomes to promote immunosuppressive effects of MDSCs

Fig. 6

a Protein level of SETD7, cyclinD1, P27, p-Akt, Akt in U87MG, U251, and P3 cells transfected with miR-1298-5p mimics and pcDNA3.1-SETD7 or pcDNA3.1 were assessed by western blotting. β-actin was used as the control for normalization. b Cell cycle analysis for U87MG and U251 cells. c–e Amounts of protein determined by densitometry of protein bands from three experiments. β-actin was the loading control. f, g The percentage of cells transfected with miR-1298-5p mimics arrested in the G1/S phase can be attenuated by SETD7 overexpression. h–k The proliferation capacity of U87MG and U251 cells treated as described above were assessed using CCK8 assay and EDU assay. Data are shown as the mean ± SD of three independent experiments. Statistical significance was determined using one-way ANOVA test (*P < 0.05; **P < 0.01; ***P < 0.001).

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