Fig. 7: The effects of miR-1298-5p overexpression in MDSCs could be partially attenuated by MSH2 overexpression.

a, b MDSCs transfected with miR-1298-5p mimics and pcDNA3.1-MSH2 or pcDNA3.1 were assessed by Flow cytometry assay. c, d The protein level of MSH2, p-p65 and p65 in MDSCs treated as described above were assessed by western blotting. Amounts of protein determined by densitometry of protein bands from three experiments. β-actin was the loading control. e The qRT-PCR assay showed the change of the expression of NOS2 and TGF-β in MDSCs treated as described above. f, g NO and TGF-β in the supernatants of MDSCs treated as described above. h, i CD8 + T cell proliferation was determined by flow cytometry 3 days later with CFSE dilution. j Schematic model showing that glioma selectively sorted oncosuppressor miR-1298-5p into exosomes and exosomal miR-1298-5p could promote the Immunosuppressive effects on of MDSCs. Moreover, miR-9-5p could promote glioma progression and induce M1 polarization of macrophages. Therefore, miR-9-5p was trapped inside cells. Data are shown as the mean ± SD of three independent experiments. Statistical significance was determined using one-way ANOVA test (*P < 0.05; **P < 0.01; ***P < 0.001).