Fig. 1: BMSC-EVs inhibit renal fibrosis in vitro and in vivo. | Cell Death & Disease

Fig. 1: BMSC-EVs inhibit renal fibrosis in vitro and in vivo.

From: Bone marrow mesenchymal stem cell-derived extracellular vesicles containing miR-181d protect rats against renal fibrosis by inhibiting KLF6 and the NF-κB signaling pathway

Fig. 1

A RT-qPCR measurement of Col4α1 expression in HK-2 cells after treatment with TGFβ-1 and BMSC-EVs. B RT-qPCR measurement of α-SMA expression in HK-2 cells after treatment with TGFβ-1 and BMSC-EVs. C RT-qPCR measurement of TGF-βR1 expression in HK-2 cells after treatment with TGFβ-1 and BMSC-EVs. In the panels of (A, B, C) *p < 0.05 vs. control. #p < 0.05 vs. TGFβ-1. D Masson’s trichrome staining for the deposition of collagen in the renal tissues from UUO rats treated with BMSC-EVs (n = 8). E Immunohistochemistry assay of Col4α1, α-SMA, TGF-βR1 and collagen I in renal tissues from UUO rats treated with BMSC-EVs (n = 8). F Western blot analysis of Col4α1, α-SMA, TGF-βR1, and collagen I in renal tissues from UUO rats treated with BMSC-EVs. In the panels of (E, F) *p < 0.05 vs. rats receiving sham operation and injected with PBS. #p < 0.05 vs. rats undergoing UUO surgery and injected with PBS. The cell experiment was repeated three independent times.

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