Fig. 5: KLF6 promotes fibrosis of HK-2 cells by activating the NF-κB signaling pathway. | Cell Death & Disease

Fig. 5: KLF6 promotes fibrosis of HK-2 cells by activating the NF-κB signaling pathway.

From: Bone marrow mesenchymal stem cell-derived extracellular vesicles containing miR-181d protect rats against renal fibrosis by inhibiting KLF6 and the NF-κB signaling pathway

Fig. 5

A PPI network of KLF6 and its top ten related genes constructed using the String website. The redder the color of the gene, the higher the degree of correlation; the bluer the color of the gene, the lower the degree of correlation. B A diagram of the correlation between KLF6 and NFKB1 expression. C A box line diagram of NFKB1 expression data from the GSE36496 dataset. The blue box on the left represents the expression in normal control samples and the red box on the right represents the expression in renal fibrosis samples. D The transduction efficiency of oe-KLF6 and sh-KLF6 as verified by RT-qPCR in HK-2 cells. *p < 0.05 vs. oe-NC. #p < 0.05 vs. sh-NC. E The effect of KLF6 on the mRNA expression of α-SMA, Col4α1, and TGF-β in HK-2 cells as detected by RT-qPCR. *p < 0.05 vs. oe-NC. #p < 0.05 vs. sh-NC. F Western blot analysis of α-SMA, Col4α1 and TGF-β protein expression in HK-2 cells with KLF6 overexpression or knockdown. *p < 0.05 vs. oe-NC. #p < 0.05 vs. sh-NC. G Western blot analysis of IκBα phosphorylation and α-SMA, Col4α1, and TGF-β protein expression in HK-2 cells after treatment with KLF6 overexpressing vector and BAY-11-7082. *p < 0.05 vs. NC. #p < 0.05 vs. TGF-β-1 + KLF6. The cell experiment was repeated three independent times.

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