Fig. 3: CD4+ T cells enhanced the function of CIK cells during ex vivo expansion. | Cell Death & Disease

Fig. 3: CD4+ T cells enhanced the function of CIK cells during ex vivo expansion.

From: CD4+ T cells are required to improve the efficacy of CIK therapy in non-small cell lung cancer

Fig. 3

A Schematic diagram depicting the ex vivo expansion of different CIK cells (left); flow cytometric quality assessment of CIK cells when ex vivo expansion at day 14 (right). B Flow cytometric examination of immune checkpoint receptors expression in CD3+CD8+ T cells. C Flow cytometric analysis of IFN-γ and GzmB production in CD3+CD8+ T cells. D Flow cytometric examination of the percentage of CD3+CD8+CD56+ T cells in CD3+CD8+ T cells. E Flow cytometric analysis of IFN-γ, GzmB, and Tim-3 expression in CD8+CD56+ T cells. F The cytotoxicity of CIK, CD4+ T, or CD4−CIK cells against A549, H520, and K562. Error bars indicate SEM, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (one-way ANOVA or Student's t test).

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