Fig. 1: DENND2D is more highly expressed in adjacent normal tissues than colorectal cancer tissues, confirmed by online database and our clinical samples.
From: Stage IV colon cancer patients without DENND2D expression benefit more from neoadjuvant chemotherapy
A Expression level of DENND2D across 31 TCGA cancer types, in comparison with their normal controls if available. The middle line in the box is the median, the bottom and top of the box are the first and third quartiles, the whiskers extend to 1.5 IQR of the lower quartile and the upper quartile respectively, and the black solid circles represent outliers. P values were derived from two-sided Wilcoxon’s rank-sum test. B DENND2D gene expression among normal, tumor, and metastatic tissues in colorectal cancer patients obtained from the TNMplot.com web tool. C Protein expression of DENND2D in colon cancer tissues from 181 stage IV CRC patients. The level of DENND2D was classified as negative, weak, moderate, and strong. Protein expression of DENND2D in normal tissue (×40 and ×200). D Protein expression of DENND2D in normal tissues. E, F Western blot analysis of six pairs of tumor tissues and normal tissues from six colon cancer patients.
