Fig. 3: DENND2D suppressed tumor proliferation, migration and colony formation in vitro.
From: Stage IV colon cancer patients without DENND2D expression benefit more from neoadjuvant chemotherapy
A, B DENND2D was knockdown by siRNA in HCT116, HT29 and SW620 confirmed by qPCR and western blot. C, D CRC cell proliferation was promoted by DENND2D knockdown. E, F CRC cell migration was promoted by DENND2D knockdown. G–I CRC cell proliferation was promoted by DENND2D knockdown. J, K DENND2D was knocked down by shRNA in HCT116 confirmed by qPCR, western blot, and IHC. L, M CRC cell proliferation was promoted by DENND2D knockdown. N CRC cell migration was promoted by DENND2D knockdown. O, P DENND2D was overexpressed in CRC cells by shRNA confirmed by qPCR, western blot, and IHC. Q, R CRC cell proliferation was suppressed by DENND2D overexpression. S CRC cell migration was suppressed by DENND2D overexpression. T, U CRC cells are more sensitive to 5FU after DENND2D knockdown.
