Fig. 5: Overexpression of KLF4 Attenuated senescence in AECs and KLF4 regulate TERT.
From: KLF4 regulates TERT expression in alveolar epithelial cells in pulmonary fibrosis
Plko.1 plasmid was used to construct KLF4 overexpression vectors. A Western blotting was performed to evaluate the expression of P21, KLF4 and TERT expression in overexpression group and control group in BEAS-2B cells. B Telomerase activity was detected by Telomerase Repeated Amplification Protocol (TRAP). C Relative telomere lengths were tested in overexpression group and control group in MLE-12 cells. D Western blotting was performed to evaluate the expression of P21, KLF4 and TERT expression in overexpression group and control group in MLE-12 cells. E Quantification of the protein level of P21, KLF4 and TERT in KLF4 overexpression group and control group in MLE-12 cells. F Relative telomere lengths were tested in MLE-12 cells. G Chromatin immunoprecipitation sequence (ChIP-seq) results showed that KLF4 can bind to TERT promoter motif. H, I ChIP-QPCR results indicated that the transcription of KLF4-bound TERT promoter was reduced in bleomycin-induced group and si-RNA group compared with control group. *P < 0.05, **P < 0.01, ***P < 0.001 by t-test.
