Fig. 3: CircLIFR suppresses HCC progression in vivo. | Cell Death & Disease

Fig. 3: CircLIFR suppresses HCC progression in vivo.

From: CircLIFR suppresses hepatocellular carcinoma progression by sponging miR-624-5p and inactivating the GSK-3β/β-catenin signaling pathway

Fig. 3

A, B Image of subcutaneous tumors in nude mice after injection of Huh7 cells (n = 6 for each group). C The tumor volume is shown to be significantly decreased in Huh7 circLIFR-overexpressing group compared with that in control group. D The tumor weight is significantly lower in circLIFR-overexpressing group than that in control group. E Representative images of xenografts stained with Ki-67 by immunohistochemistry (IHC). Images at ×200 (left panel) and ×400 (right panel) magnification. Scale bar: left = 100 μm; right = 50 μm. F Representative images of orthotopic xenograft tumor growth over time and quantitative analysis of the relative fluorescence intensity over time in Huh7 vector and circLIFR overexpression (OE-circLIFR) groups (n = 3 for each group). G Photographs of orthotopic xenograft tumor from Huh7 vector and OE-circLIFR groups and matched xenograft tumor hematoxylin and eosin (H&E) staining are shown. Images were photographed at ×100 (left panel) and ×400 (right panel) magnification. Scale bar: left = 200 μm; right = 50 μm. Data represent means ± SD of three independent experiments. *P < 0.05.

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