Fig. 4: CircLIFR can bind to miR-624-5P by serving as miRNA sponges. | Cell Death & Disease

Fig. 4: CircLIFR can bind to miR-624-5P by serving as miRNA sponges.

From: CircLIFR suppresses hepatocellular carcinoma progression by sponging miR-624-5p and inactivating the GSK-3β/β-catenin signaling pathway

Fig. 4

A qRT-PCR assay to quantify the subcellular localization of circLIFR in Huh7 and SNU387 cells. B Fluorescence in situ hybridization shows circLIFR is mainly located in the cytoplasm. C RNA immunoprecipitation (RIP) assay demonstrating that circLIFR can sponge miRNAs by using the antibody of Ago2. D Schematic illustration showing the potential target miRNAs of circLIFR, predicted by miRanda and starbase. E RNA pull-down assay shows the miRNAs enriched in Huh7 cells. F–H The luciferase activities of circLIFR-WT, circLIFR-MUT1, and circLIFR-MUT2 plasmid luciferase reporters after co-transfection with miR-624-5p mimics or mimic NC in HCC cells. Scale bar: 10 μm. Data represent means ± SD of three independent experiments. *P < 0.05.

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