Fig. 1: Expression of Tim-3 on CD14+ cells during human early pregnancy. | Cell Death & Disease

Fig. 1: Expression of Tim-3 on CD14+ cells during human early pregnancy.

From: Tim-3+ decidual Mφs induced Th2 and Treg bias in decidual CD4+T cells and promoted pregnancy maintenance via CD132

Fig. 1

A Flow cytometric analysis and quantification of frequency of Tim-3 expression on gated CD14+ cells from peripheral blood mononuclear cells (PBMCs) isolated from non-pregnant women of childbearing age (n = 9), and from paired PBMCs and decidual immune cells (DICs) during human first trimester pregnancy (n = 48). ***p < 0.001. B Quantification of flow cytometric analysis of Tim-3 expression on peripheral CD14+ (pCD14+) cells isolated from non-pregnant women or pregnant women, with or without co-culture with trophoblasts (Tros) for 48 h. n = 20. ***p < 0.001. C Quantification of flow cytometric analysis of Tim-3 expression on dCD14+ cells cultured alone or co-cultured with equal numbers of Tros (directly or indirectly), or decidual stromal cells (DSCs), or human HTR8/SVneo cells. The α-HLA-G/HLA-C antibodies were used in some wells. *p < 0.05, **p < 0.01, compared with the control. #p < 0.05, ##p < 0.01, compared with the group co-cultured with Tros. Data represent the mean ± standard error of the mean (SEM). The flow cytometry plots are representative of three independent experiments.

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